The Gibson Assembly® Ultra Kit

The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

The Gibson Assembly® Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency and constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly® Ultra reactions.

Summary

  • Multiple Inserts — Assembles up to 15 inserts at one time
  • Simple Two-Step Process — 80 minute reaction with less than 6 minutes hands-on time
  • Optimal for widely varying fragment sizes from 100 bp - 100 kb in length
  • Robust and Efficient — Complex assemblies of 95% cloning efficiencies
  • Large Constructs — Create constructs up to 1000 kb in length in multi-stage assembly reactions
  • Assembles without needing restriction enzyme sites
  • Utilize any vector using simple design strategies
  • Seamless — Constructs are joined without the presence of intervening and extraneous sequences
  • Adaptability — Easily adapted for multiple DNA manipulations,
    ss oligonucleotides and assembling repetitive sequences
  • Cloning Specificity — Enhance cloning specificity of PCR products due to homology matching between amplicon and vector
  • Frequently published method — Since 2009, the Gibson Assembly® method has been cited by over 1,200 scientific publications

How It Works

The Gibson Assembly® Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency. Constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly® Ultra reactions.

To assemble DNA using the Ultra method:

  1. Design DNA fragments with overlap regions
  2. Combine the fragments and Ultra Master Mix A==> Master Mix A catalyzes 3’ chew back
  3. Inactivate Ultra Master Mix A at 75°C
  4. Anneal DNA fragments at 60°C
  5. Combine the fragments and Ultra Master Mix B==> Master Mix B catalyzes repair

The resulting construct is double stranded, fully sealed, and can be used immediately for downstream molecular biology applications

Applications

  • Multiple Insert Cloning – Clone multiple inserts into a vector without relying on the availability of restriction sites
  • Large Fragment Assemblies – Assemble gene clusters and genome-size fragments
  • Mutagenesis – Site-directed mutagenesis to make simultaneous changes in a single reaction
  • See now the team at JCVI created the first synthetic cell using Gibson Assembly®
  • Application Notes

Gibson Assembly® Ultra Provides High Cloning Efficiency

Gibson Assembly Ultra Kit Cloning Efficiency Chart
Gibson Assembly® Ultra Kit has been designed to assemble multiple fragments in a simple two-step master mix reaction.

Gibson Assembly® Reagents

Products Catalog # Price
Gibson Assembly® Ultra Starter Kit GA1200-S $85
Gibson Assembly® Ultra Kit, 10 rxn GA1200-10 $169
Gibson Assembly® Ultra Kit, 50 rxn GA1200-50 $680
Gibson Assembly® Ultra Master Mixes (2X), 10 rxn GA1200-10MM $159
Gibson Assembly® Ultra Master Mixes (2X), 50 rxn GA1200-50MM $630
Gibson Assembly® Positive Control GA1000-05C $63

Bulk Packaging

Gibson Assembly® Ultra MasterMixes (2X)

MasterMix Volume  
MasterMix A MasterMix B Product Catalog # Total # of reactions Standard Price Discount Discounted Price Price/Reaction
5 mL 10 mL Gibson Assembly® Ultra MasterMixes (2X) GA1200-B05 1000 $12,600 52.38% $6,000 $6.00
10 mL 20 mL GA1200-B10 2000 $25,200 56.35% $11,000 $5.50
20 mL 40 mL GA1200-B20 4000 $50,400 60.32% $20,000 $5.00
30 mL 60 mL GA1200-B30 6000 $75,600 64.23% $27,000 $4.50
40 mL 80 mL GA1200-B40 8000 $100,800 68.25% $32,000 $4.00
50 mL 100 mL GA1200-B50 10000 $126,000 72.22% $35,000 $3.50

Above mentioned pricing is valid only in the U.S. For international pricing please contact customerservice@sgidna.com.

Prices do not include shipping and handling. Submit by 2pm (PST) to receive order by the next day. For bulk packaged size, lead time is ~3 weeks.

Gibson Assembly method is also available under commercial license, please inquire at info@sgidna.com

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