Minimal synthetic cells are engineered cell lines with genomes smaller than that of any autonomously replicating cell found in nature. Syn 2.0™ and Syn3.0™ Minimal Synthetic Cells are bacterial cell lines based on the genome of Mycoplasma mycoides subspecies capri strain GM12. These cell lines are derived from this naturally occurring bacterium, but have a reduced genome size that maintains genes essential for viability and growth (1). Details on the methods used to generate these cells and the properties of these cells can be found in reference 1. Each lot of these cells is tested for viability and sequenced to ensure consistency with the published genome sequence.
|Syn2.0™ Minimal Synthetic Cell||Syn3.0™ Minimal Synthetic Cell|
|Parent Bacterial Strain||Mycoplasma mycoides subspecies capri strain GM12||Mycoplasma mycoides subspecies capri strain GM12|
|Approximate Genome Size||576 kb||531 kb|
|Approximate Doubling Time||~92 min||~180 min|
|Known Protein Coding Genes||479||439|
|Known RNA-coding Genes||38||35|
|Total Number Known Genes||517||474|
|Biosafety Level (BSL)*||2||2|
|Storage||-80° C||-80° C|
Although this product poses minimal hazard to humans as the cells are not able to grow outside of carefully controlled laboratory conditions, handle Syn2.0™ and Syn3.0™ cells according to Biosafety Level 2 (BSL 2) precautions. Only those personnel with appropriate training and experience should handle this cell line.
Biosafety classification is based on U.S. Public Health Service Guidelines. Detailed presentations of biosafety in microbiological and biomedical laboratories according to the United Sates Center for Disease Control can be found by clicking here. It is the responsibility of the user to ensure that their facilities comply with biosafety regulations for their own country. Refer to your country and institute specific guidelines for details on appropriate BSL‑2 procedures.
1. C.A. Hutchison III et al. Design and synthesis of a minimal bacterial genome. Science. Vol. 351, March 25, 2016, p.1414. doi: 10.1126/science.aad6253 (link).
Cultures are grown at 37°C in SP-4 liquid medium (containing 17% fetal bovine serum), pH 6.3–6.8, without agitation, or on solid medium of the same composition, supplemented with 1% agarose. Note that these cells have a longer doubling time as compared to the parent Mycoplasma mycoides. For complete details on the creation and growth of these cells refer to C.A. Hutchison III et al. Science. Vol. 351, March 25, 2016, p.1414 (link) and the supplementary materials.
Verification of an institutes agreement and ability to comply w with BSL-2 (Biosafety Level 2) procedures may be required prior to shipment. For shipments within the United States, a USDA VS (United States Department of Agriculture Veterinary Science) Permit is required and evidence of a current USDA VS permit must be provided prior to shipment. Institutes located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to SGI-DNA in advance of shipment. International shipments will require recipient institution to verify that appropriate import permits are either in place or not required for their country.
Syn2.0™ and Syn3.0™ Minimal Cells are sold for research use only. To inquire about licensing for commercial use please inquire at firstname.lastname@example.org.
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