One of the essential technologies used by SGI-DNA is the Gibson Assembly® method, a revolutionary method developed by Daniel Gibson and colleagues at the J. Craig Venter Institute (JCVI).
Since its introduction to the life science community in 2009, the Gibson Assembly® method has become a mainstay in many synthetic biology laboratories and has attracted interest from both the academic and commercial life sciences community due to its ease-of-use, robustness and flexibility.
For non-commercial use, SGI-DNA Gibson Assembly® reagents do not require a license and there are never any royalties due. Contact us at firstname.lastname@example.org with any questions and for licensing the Gibson Assembly® method for commercial use. SGI-DNA is the only provider of Gibson Assembly® commercial licenses, and charges a yearly fee with no royalties. We strive to make Gibson Assembly® easy and convenient to use for all scientific researchers.
Find out why scientists prefer the Gibson Assembly® method – Watch our video!
|Feature||Gibson Assembly® HiFi 1-Step Kit||Gibson Assembly® Ultra Kit||Gibson Assembly® Site Directed Mutagenesis Kit|
|Hands on Time||<5 minutes||<6 minutes||<40 minutes|
|Reaction Time||60 minutes||80 minutes||4-6 hours|
|No. of steps||1||2||3|
|Size of each fragment||500bp to 32kb||100bp to 100kb||500bp to 5.5kb|
|Efficiency||>90%||>95%||>95% for single site >55% for five sites|
|No. of fragments you can assemble||Up to 5||Up to 15||Up to 5|
|Total construct size||100kb (in multi stage reactions)||1000kb (in multi stage reactions)||50kb (in multi stage reactions)|