“In the field of protein NMR… the costs of labeled media can reach thousands of dollars using conventional E.coli strains for recombinant protein expression. Using V. natriegens Vmax™ cells, we can reduce those costs to about half due to the 2–4X higher expression yields. The time needed until harvesting the cells can be reduced significantly because of the ability to induce Vmax™ cells before reaching exponential phase.” | Klaus Zangger — University of Graz
The only next-generation bacterial expression system
Vmax™ X2 cells are derived from one of the fastest growing organisms on earth: the gram-negative, non-pathogenic marine bacterium, Vibrio natriegens.
Vmax™ cells double two times faster than E. coli and generate significantly greater amounts of biomass and protein per volume of cells.
Simply stated, Vmax™ X2 cells result in much larger amounts of soluble protein, much faster.
Join us in saying #GoodbyeEcoli
VMAX™ X2 COMPETENT CELLS
A new host system with advantages over E. coli
Vmax™ X2 competent cells are engineered to retain the benefits of traditional bacterial protein expression systems — low cost, easy to grow, high transformation efficiency, and compatible with plasmids and antibiotics already in widespread use.
With an accelerated doubling time, Vmax™ X2 cells shorten protein expression workflows by one day and generate more soluble protein in less time than E. coli.
Additionally, Vmax™ X2 cells naturally have low immunogenicity with endotoxin levels 100 times lower than E. coli, making them an ideal host system for protein purification.
Incorporates into your existing workflow
Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21 (DE3) and have a transformation efficiency rivaling the best alternative protein expression strains. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ X2 cells can be induced over a wide range of timepoints, from an OD600 of 0.2 to over 1.0, without loss of final protein yield, dramatically increasing workflow flexibility.