Multi-Tile Assembly and Cloning

in your lab on the BioXp™ 3200

> Eliminate sub-cloning completely

> Build complex structures

> Maximize throughput with a plate-based format

> Rapidly iterate DNA design

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Make discoveries in days, not months

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Assemble Larger Complex DNA Constructs

The first fully automated seamless cloning strategy that allows for the scarless insertion of multiple DNA fragments into multiple expression vectors. SGI-DNA’s BioXp™ 3200 System has adapted Gibson Assembly® Technology to produce a variety of multi-fragment assembly combinations in less than 15 hours, enabling BioXp instrument users to generate large multi-variant constructs, cloned into single or multiple vectors. BioXp MTAC sequences range in size between 300 and 1800 bp, which can be combined for a length up to 3600 bp. Constant regions of virtually any length can also be cloned into the designed plasmid. Harnessing the ease of scarless Gibson Assembly® Method, the MTAC kit allows flexible design capabilities for a variety of downstream applications.

Antibody Engineering

Flexible design options of variable heavy chain and light chain sequences produce antibody variants, including multicistronic and multi-specific antibody constructs. Constructs can be combined with your constant backbone or linker and cloned into desired vectors.

Synthetic Biology

The BioXp™ MTAC Kit provides control and flexibility, automating assembly of complex sequences up to 3.6 kb for multiple downstream formats.