Multi-tile assembly and cloning
For use in your lab using the BioXp™ 3200 system
Eliminate sub-cloning completely
Build complex structures
Maximize throughput with a plate-based format
Rapidly iterate DNA design
Make discoveries in days, not months
Using the BioXp™ multi-tile assembly and cloning (MTAC) kit, you have the power to design, build, test, and iterate rapidly. Take complete control over your genetic engineering and antibody discovery timelines and make discoveries in days, not months.
Using MTAC significantly increases time to discovery whether you are doing cloning on the bench or using service labs.
Your time is precious and your work is too important to wait.
Assemble larger complex DNA constructs
The first fully automated seamless cloning strategy that allows for the scarless insertion of multiple DNA fragments into multiple expression vectors.
SGI-DNA’s BioXp™ 3200 system has adapted Gibson Assembly® technology to produce a variety of multi-fragment assembly combinations in less than 15 hours, enabling BioXp™ instrument users to generate large multi-variant constructs, cloned into single or multiple vectors. BioXp™ MTAC sequences range in size between 300 and 1800 bp, which can be combined for a length up to 3,600 bp. Constant regions of virtually any length can also be cloned into the designed plasmid.
Harnessing the ease of the scarless Gibson Assembly® method, the MTAC kit allows flexible design capabilities for a variety of downstream applications.
Flexible design options of variable heavy chain and light chain sequences produce antibody variants, including multicistronic and multi-specific antibody constructs. Constructs can be combined with your constant backbone or linker and cloned into desired vectors.
The BioXp™ MTAC kit provides control and flexibility, automating assembly of complex sequences up to 3.6 kb for multiple downstream formats.