SGI-DNA's Gibson Assembly® Ultra reagent kit provides error correction with the longest, high-fidelity builds available.
Gibson Assembly® cloning is the gold standard for cloning routine and complex DNA assembly in laboratories around the world because it is seamless, faster, and more robust than all other cloning methods.
Gibson Assembly® RapidAMP™ takes it one step further and provides synthesized, transfection-ready quantities of DNA in an endonuclease-free reaction.
Gibson Assembly® advantages:
Use simple, robust protocols
Seamlessly assemble more fragments
High-fidelity, error-corrected assemblies
Build large, complex constructs easily
Scale up with the BioXp™ 3200 system
Gibson Assembly® reagents are available in a benchtop reagent kit or in automated format, compatible with the BioXp™ 3200 system. The BioXp™ system enables up to 24 constructs to be built, cloned, and amplified in a single overnight run, and completely eliminates the need to transform into E.coli.
The BioXp™ system easily synthesizes the required fragments, up to 24 at a time, and then has the ability to clone them into the vectors of interest (up to 4) in an overnight run on the instrument. By the following afternoon, these clones can be amplified to > 10 µg DNA using Gibson Assembly® RapidAMP™ technology.
Only with the BioXp™ system is it possible to shorten the design-build-test cycle to just two days.