Build and amplify complex DNA constructs quickly
The benchtop RapidAMP™ process begins with a linearized vector plus up to three linear DNA insert fragments, 300-1800 bp each. All are assembled via Gibson Assembly® technology to generate a circular cloning product.
A robust amplification workflow
The RapidAMP™ workflow allows microgram amounts of specific DNA constructs in a few hours
The Gibson Assembly® RapidAMP™ workflow produces clean, high-yield DNA material. A representative gel shows clean bands post-restriction digest and before any DNA purification. After cloning and amplification on the bench, or with the BioXp™ 3200 system, the final DNA product can be digested to linear form and is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. RapidAMP™ has been used successfully for transfection (lipofection and electroporation) and in vitro transcription.
Cloning without E.coli — Gene assembly and amplification of up to 24 full endotoxin-free constructs
Scale up with the BioXp™ 3200
Gibson Assembly® RapidAMP™ reagents are available in a benchtop reagent kit or in automated format, compatible with the BioXp™ 3200 System. The BioXp™ enables up to 24 constructs to be built, cloned, and amplified in a single overnight run, eliminating the need to transform into E. coli.
The BioXp™ system starts by synthesizing the required fragments, up to 24 at a time, and then clones them into the vectors of interest (up to 4) in an overnight run, on the instrument. By the following afternoon, these clones will have been amplified to > 10 µg DNA using Gibson Assembly® RapidAMP™ technology. Only with BioXp™ System is it possible to shorten the design-build-test cycle to just two days.
RapidAMP™ workflows vs. traditional cloning
Save over a week (from two to nine days)