Build and amplify complex DNA constructs overnight
The BioXp™ 3200 system enables up to 24 constructs to be built, cloned, and amplified in a single overnight run, eliminating the need to transform into E. coli. The BioXp™ system starts by synthesizing the required fragments, up to 24 at a time, and then clones them into the vectors of interest in an overnight run on the instrument. These clones will be amplified to > 10 µg DNA using Gibson Assembly® RapidAMP™ technology.
A robust amplification workflow
The Gibson Assembly® RapidAMP™ workflow produces clean, high-yield DNA material. A representative gel shows clean bands post-restriction digest and before any dna purification. After cloning and amplification on the BioXp™ 3200 system, the final DNA product can be digested to linear form and is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. The RapidAMP™ workflow has been used successfully for transfection (lipofection and electroporation) and in vitro transcription.