Traditional Cloning

Traditional Restriction Digestion Cloning

  • Time consuming (multiple steps)
  • Poor efficiency
  • Can't assemble multiple inserts at once
  • Poor sequence fidelity

Gibson Assembly® HiFi 1-Step Kit

Efficient, high fidelity cloning of single or multiple DNA fragments in one simple step.

Gibson Assembly

Gibson Assembly® Cloning

  • 1 hour 1 step protocol
  • >90% cloning efficiency
  • Assemble 5 inserts in 1 step
  • >95% sequence fidelity

With Gibson Assembly® HiFi 1-Step Kit, you’re just one step away from your perfect clone.