with Gibson Assembly® reagents and our new Cloning Guide

Gibson Assembly DNA MY WAY Scientist

Get DNA your way. Generate seamless plasmid DNA or BAC clones without restriction digestion or DNA scars.

Gibson Assembly® cloning, developed by the J. Craig Venter Institute and Synthetic Genomics, allows for insertion of DNA fragments into virtually any vector without the need for compatible restriction sites. Join any two fragments to generate seamless constructs in your desired vector.

  • Significantly faster than traditional cloning methods
  • Avoid time-consuming subcloning
  • Insert 1-15 fragments in a single round of cloning in ~1 hour
  • Achieve cloning efficiencies of up to 95%

Gibson Assembly® Cloning Kits and Reagents are available through VWR or from our website:

Get #DNAMYWAY by Downloading The New Gibson Assembly® Cloning Guide

Whether you're new to Gibson Assembly® cloning, or an experienced user, you'll benefit from our 40-page cloning guide which describes the benefits, applications, and protocols:

  • Gain perspective on this revolutionary seamless cloning technology from a short history and foreword from Synthetic Genomics' Dan Gibson
  • Learn useful tips and tricks such as:
    • How to use SGI-DNA's Gibson Assembly® Primer Design Tool
    • The best kit to use for different fragment size/overlap situations
  • Discover new applications for Gibson Assembly® such as PBnJ Cloning, which does not require homologous ends

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