Custom DNA Cloning using Gibson Assembly | SGI-DNA


Custom, Error-Free, Synthetic DNA Clones Ready for Your Downstream Application

From simple 125 bp constructs to large complex constructs up to 2 Mbp in size, SGI-DNA has the expertise to deliver an error-free clone custom made to meet your requirements. With internal capabilities born out of the J. Craig Venter Institute and Synthetic Genomics, Inc., our proprietary error-correction technology and patented Gibson Assembly® method provide us the unique ability to take on your most challenging gene synthesis projects.

All constructs are made to your exact specifications. Submit your sequences to get a competitive price quotation. Minimum order $300.


  • Error-free gene synthesis – 100% sequence accuracy guaranteed
  • Standard genes up to 15 kb
  • Large and complex genes up to 2 Mb
  • Clones provided in our standard pUC or BAC vector or your custom vector
  • Sequence data delivered as annotated Genbank files with a plasmid map
  • Trace files, raw sequence files, sequencing of vector available upon request
  • Codon expression optimization service available
  • Large scale expression, DNA normalization services also available
SGI-DNA Cloning

SGI-DNA routinely synthesizes DNA constructs greater than 100kb. Examples of these large and complex synthesis capabilities include the creation of the 1.1 Mbp Mycoplasma genome and rapid, robust production of synthetic vaccine viruses (Synthetic Genomics Vaccines, Inc.). Laboratories count on us when synthesizing complex sequences such as those with high and low GC content, tandem repeats, homopolymers and palindromes. Our California-based synthetic biology experts are committed to producing DNA clones quickly and economically to save you time and money.

Gene Synthesis of DNA Clones Workflow

Gene Synthesis of DNA Clones Workflow

Large Constructs

Synthesis of a 1.1 Mbp Construct. SGI-DNA’s technology has delivered the largest synthetic construct to date: the 1.1 Mbp Mycoplasma Genome. (Science, 2008 Gibson et al., 319 (5867): 1215-1220)

Complex or Difficult Sequences

Successful Synthesis of 20 x 22 bp Identical Repeat Construct. We have experience cloning and synthesizing complex sequences such as this error-free 512 bp clone consisting of 20 x 22bp identical repeats.

  Standard Genes Large and Complex Genes
Length Up to 15 kb Up to 2 million bp
Fidelity Sequence verified Sequence verified
Complexity - High or low GC Repeats
Custom Vector Available Available
Delivery Time Starts at 10-15 days Inquire

From simple constructs to large and complex constructs, our goal is to provide you the plasmid or BAC clones you need to move your projects forward. Our team of synthesis experts is dedicated to delivering error-free clones in the format you require.

Standard Services Provide for All Constructs

  • Complexity analysis prior to synthesis
  • Error free cloning into our standard vectors (custom vector service available)
  • Codon optimization (upon request)
  • DNA sequencing to confirm creation of error-free construct
  • Delivery of minimum of 200 ng of plasmid

Additional Services Available

  • Cloning into custom vectors
  • Large scale plasmid preparation
  • Normalization of concentration or amounts
  • Custom vialing/packaging
  • Glycerol stock preparation
  • cGMP synthesis

Length (kb) Delivery Time*
0.125-3.0 10-15 Business Days
3.0-6.0 12-15 Business Days
6.0-15.0 12-20 Business Days
15-50 20-30 Business Days
50-96 30-40 Business Days
96-200 Inquire

Please contact us for pricing for your specific constructs. Minimum order $300.

Deliverables for DNACLONES

Product Specifications

Size Ranges From 125 bp to 2 million bp
Fidelity 100% sequence accuracy guaranteed
Composition A, T, C or G residues

Synthesized into SGI-DNA vector (modified pUC or BAC). Inquire for custom vectors.

Amount Delivered

< 20 kb in BAC: 200 ng
< 20 kb in pUC: 500 ng
> 20 kb in BAC: 500 ng

Format Shipped dry in tubes or 96 well plate
Quality Assurance

100% sequence verification using Next Generation and Sanger sequencing methods.

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