Vmax™ X2 is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. Vmax™ cells are derived from the gram-negative, low endotoxin, non-pathogenic marine bacterium, Vibrio natriegens. With a doubling time twice that of E. coli and the ability to generate greater amounts of biomass per volume of cells, Vmax X2 results in larger amounts of soluble protein faster.
Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3) and has a transformation efficiency rivaling the best alternative bacterial expression strains.
Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ cells can be induced over a wide range of time points, from an OD600 of 0.1 to over 1.0, without a loss of final protein yield, dramatically increasing workflow flexibility.
Vmax™ X2 is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. To obtain the fastest growth and highest protein yield, we recommend Vmax™ enriched growth medium.
A variety of commonly used media support the growth of Vmax™. However, due to the strain’s origins as the marine bacterium Vibrio natriegens, Vmax™ cultures grow best in osmotically rich media with higher salt concentrations. This nutritionally rich, optimized medium results in rapid growth and robust expression for Vmax™ cultures.