Originally pioneered by SGI-DNA’s own Dan Gibson, the Gibson Assembly method is an efficient and robust cloning workflow that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.

      SGI-DNA offers several Gibson Assembly cloning kits for high performance DNA assembly, including the popular HiFi 1-Step master mix and the Ultra 2-Step master mix for the highest cloning efficiency available and broad fragment size ranges, ideal for complex projects.

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