Gibson Assembly RapidAMP™ Ultra kit provides a single day, seamless, assembly-to-transfection-ready DNA workflow. The process requires less than six hours, starting with linear double-stranded DNA fragments prepared for Gibson Assembly® and ending with >10 ug of amplified DNA. The final DNA product can be digested to linear form and is then ready for downstream applications, negating the need for traditionalE. colitransformation and plasmid purification. The RapidAMP™ product has been used successfully for transfection (lipofection and electroporation) andin vitrotranscription. The RapidAMP™ product is mostly linear, with the balance consisting of concatemers and some circular DNA material. The degree of linearization can be controlled by restriction digestion, time, and temperature.