The BioXp™ 3200 System automates the construction of next generation sequencing libraries. This genomic workstation provides a simple process that eliminates the time, hassle, and potential for variability that can occur with manually prepared NGS libraries. Built for Illumina® sequencing platforms, the BioXp™ 3200 NGS library construction method allows the construction of up to 16 barcoded libraries starting with 10 ng to 50 ng of input genomic or plasmid DNA. This kit is also available in a non-automated format.
The BioXp™ System NGS Library Construction Kits offer:
Built for ease of use, even novice scientists can successfully prepare a library ready for Illumina NGS sequencing. Simply add sheared DNA sample to the sample plate, load the reagents on the BioXp™ deck and press start.
The BioXp™ system runs hands-free to create barcoded NGS libraries ready for normalization, pooling, and sequencing in under 5 hours. The reliable NGS library construction chemistry efficiently adds to adapters to input DNA samples from 29% to 68% GC content to produce large numbers of unique sequencing reads.
Using the BioXp™ system and the NGS Library Construction Kits, you can consistently build reliable NGS libraries. This easy-to-use system is ideal for labs needing automated low to medium throughput of NGS library preparation. The BioXp™ system is a complete genomic workstation that enables other molecular biology applications including generation of synthetic double stranded DNA fragments and automated cloning. Learn more about the BioXp™ 3200 System.
Not ready for automation? Want to compare this library construction method to your present method? The BioXp™ NGS Library Construction Kit is also available as a manual library construction format. In either case our optimized enzymes and buffers facilitate the ligation of the provided barcode adapters resulting in diverse sequencing reads. In either an automated or manual format, these complete NGS library construction kits include barcode adapters and magnetic beads so you have all the key components to ensure the highest quality libraries for reliable sequencing results.
All key reagents required to perform automated NGS library construction on the BioXp™ system are provided in the kit.
Simple and rapid set up of BioXp™ 3200 for NGS library construction. The BioXp™ 3200 System deck, with all NGS library construction reagents in appropriate location is shown. Setup for automated library construction can be accomplished by loading kit components as described here. First, add the sheared DNA sample to the sample plate and place in the location shown. Next place the pre-aliquoted NGS library construction reagents, the barcode plate, and magnetic bead strips provided with the kit, in the locations shown. Fill the ethanol reservoir and place in the location shown. Finally, add the transfer tips into the holders (tips sold separately), close the lid and press start
Sample result from BioXp™3200 NGS library construction and sequencing. Replicate NGS libraries were constructed from samples of Bacillus genomic DNA or Vibrio genomic DNA using the BioXp™ 3200 NGS Library Construction Kit and BioXp™ instrument. The average size of fragment sequenced and % dimer present in the final library and the percent library were determined using an Agilent 2100 Bioanalyzer and a High Sensitivity DNA Chip. Resulting libraries were sequenced using Illumina MiSeq® system. Total number of reads, the percentage of reads mapped to reference genome, average coverage (average number of reads that map to reference genome), and maximum coverage were determined using the CLC Genomics Workbench. Data shown indicate creation of a high-quality library that resulted in reliable sequencing data output.
Minimal GC bias for reliable results across a range of samples. GC bias for three different bacterial genomes with a range of GC content [Low, C. difficile 29% GC (left), Moderate V. natriegens 45% GC (middle) and High, B. pertussis 68% GC (right)] were used to prepare NGS libraries using the BioXp™ 3200 NGS Library Construction Kit. For each genome, the GC content of the reference genome in 100 bp bins was plotted vs. the normalized coverage across these bins using Picard CollectGCBiasMetrics. In the absence of sequencing bias, all bins would be equally represented and indicated by a horizontal distribution centered on a normalized coverage of 1. In the figure shown, distribution of GC content in the genome is indicated by the blue histograms. The normalized coverage for the BioXp™ NGS Library Construction Kit is shown by the orange line and is consistent with the level of bias reported for other commercially available kits.
*Automated reagent kit. Requires the BioXp™ 3200 System to run chemistry.
**Non-automated version of kit utilizes same enzymes, buffers, magnetic beads and barcodes as automated version
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