Generate high quality libraries and automate routine Next Generation Sequencing (NGS) sample preparation on the BioXp™ 3200 System.
Reduce bottlenecks in your NGS workflow by preparing sequencing-ready DNA libraries on your own benchtop.
This easy-to-use system is ideal for labs needing an automated solution for NGS library preparation.
The BioXp™ 3200 system is a genomic workstation enables the automation of the NGS library preps and a variety of molecular biology applications. This versatile system also functions as a DNA printer by building synthetic genes in an overnight run.
All key reagents required to perform automated NGS library construction on the BioXp™ system are provided in the kit.
Simple and rapid set up of BioXp™ 3200 for NGS library construction. The BioXp™ 3200 System deck, with all NGS library construction reagents in appropriate location is shown. Setup for automated library construction can be accomplished by loading kit components as described here. First, add the sheared DNA sample to the sample plate and place in the location shown. Next place the pre-aliquoted NGS library construction reagents, the barcode plate, and magnetic bead strips provided with the kit, in the locations shown. Fill the ethanol reservoir and place in the location shown. Finally, add the transfer tips into the holders (tips sold separately), close the lid and press start
Sample result from BioXp™3200 NGS library construction and sequencing. Replicate NGS libraries were constructed from samples of Bacillus genomic DNA or Vibrio genomic DNA using the BioXp™ 3200 NGS Library Construction Kit and BioXp™ instrument. The average size of fragment sequenced and % dimer present in the final library and the percent library were determined using an Agilent 2100 Bioanalyzer and a High Sensitivity DNA Chip. Resulting libraries were sequenced using Illumina MiSeq® system. Total number of reads, the percentage of reads mapped to reference genome, average coverage (average number of reads that map to reference genome), and maximum coverage were determined using the CLC Genomics Workbench. Data shown indicate creation of a high-quality library that resulted in reliable sequencing data output.
Minimal GC bias for reliable results across a range of samples. GC bias for three different bacterial genomes with a range of GC content [Low, C. difficile 29% GC (left), Moderate V. natriegens 45% GC (middle) and High, B. pertussis 68% GC (right)] were used to prepare NGS libraries using the BioXp™ 3200 NGS Library Construction Kit. For each genome, the GC content of the reference genome in 100 bp bins was plotted vs. the normalized coverage across these bins using Picard CollectGCBiasMetrics. In the absence of sequencing bias, all bins would be equally represented and indicated by a horizontal distribution centered on a normalized coverage of 1. In the figure shown, distribution of GC content in the genome is indicated by the blue histograms. The normalized coverage for the BioXp™ NGS Library Construction Kit is shown by the orange line and is consistent with the level of bias reported for other commercially available kits.
*Automated reagent kit. Requires the BioXp™ 3200 System to run chemistry.
**Non-automated version of kit utilizes same enzymes, buffers, magnetic beads and barcodes as automated version
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